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CELLULOSIC MATERIALS FOR BIOTECHNOLOGICAL SEPARATIONS


DANIELA SUTEU 1*, VALERIA-MARTA GORDUZA 2, CAMELIA OLTEANU 2, OVIDIU TOMA 3
(1)TECHNICAL UNIVERSITY “GH.ASACHI” IAŞI, FACULTY OF CHEMICAL ENGINEERING AND ENVIRONMENTAL PROTECTION, DEPARTMENT OF ENVIRONMENTAL ENGINEERING AND MANAGEMENT, AV.D. MANGERON, 71A, IAŞI-700050, ROMANIA
(2)TECHNICAL UNIVERSITY “GH.ASACHI” IAŞI, FACUL

Issue:

SCSCC6, Volume IX, No. 4

Section:

Volume IX, No. 4 (2008)

Abstract:

New cellulosic materials functionalized with reactive dyes and metallic ions are of interest in affinity separation of enzymes. The purpose of this work is to present a review of our research activities, concerning the opportunities of cellulosic sorbents modified with reactive dyes and/or metallic ions to use in retention of biochemical compounds. We study the retention of lysozyme onto GE and AE microcrystalline ion exchange celluloses unmodified and functionalized with dye Reactive Red 120 and/or Cu2+ ions. The retention capacities of tested materials were evaluated by value of sorption capacity calculated using Langmuir parameters. The results of this study evidenced that celluloses materials tested proved to be an effective sorbent in retaining of proteic compounds traces from aqueous media. In the same time it was established the higher capacity of cellulose ion exchange for lysozyme uptake than that of the same cellulose modified with dye and Cu2+ ions.

Keywords:

affinity separation methods, sorption, cellulosic materials, lysozyme, reactive dyes - biomimetic ligands, metallic ions.

Code [ID]:

CSCC6200809V04S01A0002 [0002608]

Full paper:

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