Quick search
Go!

DIFFERENCES BETWEEN THE DOUBLE AND SINGLE STRANDED DNA THROUGH THE RAYLEIGH LIGHT DISPERSION PHENOMENON
DIFFÉRENCES ENTRE L’ADN DOUBLE ET MONOCATÉNAIRE VUES A TRAVERS DU PHÉNOMENE RAYLEIGH DE DISPERSION DE LA LUMIERE


MIHAELA ILIE 1*, DANIELA COLTUC 2
(1) UNIVERSITÉ DE MÉDICINE ET PHARMACIE «CAROL DAVILA», FACULTÉ DE PHARMACIE, TRAIAN VUIA NO. 6, BUCAREST, ROUMANIE
(2) UNIVERSITÉ “POLITEHNICA” DE BUCAREST, FACULTÉ D’ELECTRONIQUE, TÉLÉCOMMUNICATIONS ET DE LA TECHNOLOGIE D’INFORMATION, ROUMANIE

Issue:

SCSCC6, Volume X, No. 2

Section:

Volume X, No. 2 (2009)

Abstract:

The paper presents the results of a chemometric analysis with the aim of highlighting the differences between the double- and single stranded DNA by using Rayleigh Light Scattering (RLS) spectra.

The spectra were obtained by exciting the aqueous solutions of calf thymus DNA in the presence of a molecular probe (the Terbium chelate of the diethylenetriaminopentaacetic acid – Tb-DTPA). Each spectrum consisted in 400 points representing the measured intensities in the range 200-400 nm, with a 0.5 nm step.

The paper presents two kinds of chemometric analysis: Principal Component Analysis (PCA) and Independent Component Analysis (ICA). PCA is well known for its ability in confining the information in a reduced number of uncorrelated coefficients, whilst ACI – lesser used in chemometrics – refines the analysis by imposing the condition of statistically independent components. Both PCA and ICA were used to reduce the dimension of each spectrum to two components. The two-dimensional representation of these components puts a into evidence a clusterisation of the spectra following the excitation wavelength and, for the same excitation wavelength, a separation tendency between spectra belonging to a different kind of DNA (single-stranded and double-stranded).

Keywords:

pattern recognition, Principal Component Analysis, Independent Component Analysis, Rayleigh Light Scattering, DNA.

Code [ID]:

CSCC6200910V02S01A0005 [0002633]

Full paper:

Download pdf


Copyright (c) 1995-2007 University of Bacău