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PARTICULARITY ON LIQUID CHROMATOGRAPHIC SEPARATION FOR AN EQUIMOLAR MIXTURE OF 2,4-DINITROPHENYLHYDRAZONES PROVIDED BY ACETALDEHYDE AND DIACETYL


GHEORGHE ZGHEREA 1 *, CRISTINA STOIAN 1, SANDU PERETZ 2
1. UNIVERSITY “DUNĂREA DE JOS” OF GALATI, FACULTY OF SCIENCES, DEPARTMENT OF CHEMISTRY, 111 DOMNEASCA STREET, 800201 GALATI, ROMANIA
2. INSTITUTE OF PHYSICAL CHEMISTRY “I. MURGULESCU”, DEPARTMENT OF COLLOIDS, 202 SPL. INDEPENDENTEI, 060021, BUCHAREST, ROMANIA
Corresponding author: gzgherea@ugal.ro

Issue:

SCSCC6, Volume XII, No. 3

Section:

Volume XII, No. 3(2011)

Abstract:

An equimolar mixture of 2,4-dinitrophenylhidrazones (2,4-DNPH-ones) providing by acetaldehyde and diacetyl has been analyzed by liquid-chromatographic separation, using the mechanism of partition with reverse phase. As mobile phase are utilized various binary mixtures, containing water (0 – 45%) and methanol. By the experimental studies were identified four domains of behavior and two optimal binary mixtures, containing 25 and 45% water respectively. The peaks are characterized by values of retention time and by position. The separation processes were appreciated by difference between the retention times of peaks; if the percent of water increase, the values of retention times is higher, heaving a maxim value when the percent of water is 25%. For a mixture of mobile phase with 35% water and 65% methanol, the difference between retention time’s values became null; the behavior is comparable with those of pure methanol, but for higher values of retention times. When the percent of water is 45%, the difference between the retention times is also maxim, associated with a change of peaks positions.

Keywords:

2, 4-dinitrophenylhidrazones, acetaldehyde, diacetyl, inversion, reverses phase, polarity, percent of water.

Code [ID]:

CSCC6201103V03S01A0011 [0003387]

Full paper:

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