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APPLICATION OF RESPONSE SURFACE METHOD ON PURIFICATION OF GLUCOMANNAN FROM AMORPHOPHALLUS ONCOPHYLLUS BY USING 2-PROPANOL


DYAH H. WARDHANI *, FATONI NUGROHO, M. MUSLIHUDIN, NITA ARYANTI
University of Diponegoro, Faculty of Engineering, Department of Chemical Engineering, Jl. Prof. Sudarto SH, Tembalang, Semarang, Indonesia
*Corresponding author: dhwardhani@undip.ac.id

Issue:

SCSCC6, Volume XVII, No. 1

Section:

Volume 17, No. 1 (2016)

Abstract:

Glucomannan purification methods affect the properties of mannan and have influence to the scope of glucomannan applications. Combination between ethanol solution and thermal treatment is commonly method applied to purify glucomannan obtained from Amorphophallus sp. However, 2-propanol was reported to be more effective in removing glucomannan impurities including the starch and the carotenes. The objective of this research was to study the effect of 2-propanol concentration, temperature and time as well as their interaction on purification of glucomannan obtained from Amorphophallus oncophyllus by using response surface methods. The relevant parameters (glucomannan content, starch content, degree of whiteness, yield) were investigated in order to establish mathematical model. The results showed that the linear models were reliable to predict the responses (R2 ≥ 0.926). Temperature was a significant variable for the all responses. This purification method improved the absorbance of the functional groups at ~2900 cm-1 of stretching of C-H vibration and ~1730 cm-1 of acetyl group which is responsible on glucomannan solubility. However, this method reduced the absorbance of the functional groups at ~1650, ~1070, ~1020 and 900 - 800 cm-1 of absorbed water or protein, C-O alcohol and -glucosidic and -mannosidic linkages, respectively. The morphology observation revealed that this purification method achieves separation and removal of impurities which encapsulated the glucomannan and subsequently released the glucomannan granules. The highest content of glucomannan obtained in this study was 72.30 % when 90 % 2-propanol at 75 °C for 180 min was used for purification.

Keywords:

Amorphophallus oncophyllus, extraction, glucomannan, 2-propanol, purification, response surface methods.

Code [ID]:

CSCC6201601V01S01A0007 [0004360]

Full paper:

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