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ALKALI EXTRACTION OF POLYSACCHARIDES FROM PROSO MILLET AND ANTIOXIDANT ACTIVITY


AI S. ZHU *, MAO Q. XU, JUN Z. ZHAO
Zhejiang University of Science and Technology, School of Biological and Chemical Engineering, 318 Liuhe Road, Hangzhou, China
*Corresponding author: 939454044@qq.com, zhuaishi86@163.com

Issue:

SCSCC6, Volume XVIII, No. 4

Section:

Volume 18, No. 4 (2017)

Abstract:

The alkali extraction of polysaccharides from proso millet was investigated by Response Surface Methodology (RSM) based on a three level, four variable Box–Behnken designs in order to obtain an optimal extraction condition combination of alkali concentration, liquid-solid ratio, extraction time, and extraction temperature. The experimental results were fitted to a quadratic polynomial and analyzed. Meanwhile was the antioxidant activity of polysaccharides extracted from proso millet measured in vitro. The results showed that the optimum conditions were alkali concentration 0.87 mol‧L-1, liquid-solid ratio 23.1:1 mL‧g-1, extraction time 1.61 h and extraction temperature 77.7 °C, at this condition combination, the experimental yield of polysaccharides was 38.27 ± 0.35 mg‧g-1, which was very close to the model predicted value of 36.12 mg‧g-1. The quadratic polynomial mathematical model had a good degree of fitting with experimental data (p < 0.0001) and could there out indicate the optimal extraction process conditions. The proso millet polysaccharides were proved by the antioxidant activity experiment to have better radical scavenging activity on superoxide, hydroxyl and DPPH (1,1-Diphenyl-2-picrylhydrazyl).

Keywords:

alkali extraction, antioxidant activity, polysaccharides, proso millet, response surface methodology.

Code [ID]:

CSCC6201704V04S01A0001 [0004632]

Note:

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