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DETERMINATION OF AFLATOXINS IN RICE USING QUECHERS AND FLUORESCENCE HPLC


AHMED GOTAH, KEY WHANG, CORALIA V. GARCIA*
Keimyung University, Department of Food Science & Technology, Daegu 42601, South Korea
*Corresponding author: cvvgarcia@kmu.ac.kr

Issue:

SCSCC6, Volume XIX, No. 2

Section:

Volume 19, No. 2 (2018)

Abstract:

This study presents the determination of aflatoxins B1, B2, G1, and G2 in rice, applying a QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) extraction followed by high-performance liquid chromatography-fluorescence detection (HPLC-FLD). The objective of the proposed method is a fast, inexpensive, and easy analysis of aflatoxins. The aflatoxins were separated on a C18 column by isocratic elution with water: methanol: acetonitrile (65 : 25 : 10, v/v/v), and detection was achieved at excitation/emission wavelengths of 360/450 nm, without derivatization. The calibration curves showed good linearity (R2 > 0.99), and the limits of detection and quantification were ≤ 6 and ≤ 8 µg·kg-1, respectively. Average intraday and interday recoveries were in the range 104 - 119 % and 104 - 113 % with RSD % ≤ 12 % for concentrations between 6 - 20 µg·kg-1. The proposed method was tested on rice samples sold on markets, of which none exhibited presence of aflatoxins.

Keywords:

HPLC-FLD, mycotoxins, Oryza sativa, xenobiotics.

Code [ID]:

CSCC6201802V02S01A0003 [0004709]

Note:

Full paper:

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