SVETLANA G. DAVYDENKO 1*, TATIANA V. MELEDINA 2, VERA A. IVANOVA 2* 1. Baltika Breweries â Part of Carlsberg Group, Innovation & Research Department Easten Europe Russia HQ, Baltika Breweries Part of the Carlsberg Group, 194292, 6 Verkhny per., 3, St Petersburg, Russia
2. ITMO University, Faculty of Food Biotechnologies and Engineering, 191002, Lomonosova Street, 9, Saint-Petersburg, Russia
*Corresponding author: davydenko@baltika.com, vera_ershova@mail.ru
We present here an original express method of quantitative assessment based on carbon dioxide intensity production by yeast Saccharomyces cerevisiae. Yeast suspension is sucked inside medical syringe, hermetically sealed from one side. During yeast fermentation carbon dioxide intensively releases and the syringe pistol starts to raise. Probes, containing toxic substances show less fermentation efficiency and the height of pistol rising is less compared to the control probe, giving opportunity to assume toxic effects of different substances, foodstuff and medicines Method is independent from highly skilled personnel and hi-tech expensive equipment. By means of a simple technique it allows receiving a comparative assessment of beverages toxicity, such as beer, vodka, energy drinks, etc. and can give information on toxicity and chemical pollution of water, food, and soil. Test for toxic effect of medicines can also become a scope of this approach. According to this concept, it is necessary to replace laboratory animals by other models. Thus, the offered technique is a tool for detection of environment potential danger to humans without experimental testing on animal models. Now days there is worldwide growing tendency: people ignore cosmetic products that were tested on animals. Our method for some extend is able to replace animal models for toxicity assessment.
