Abstract: | In Romania, the pepper crops (Capsicum annuum L.) occupies an important place in agriculture. The surface occupied with this crops in 2004 was of almost 18.104 ha and the production harvested for fresh consumption was 237.240 Mt (FAO, 2005). Unfortunatelly, this important crop is affected by many pathogens (including viruses, fungi, bacteria and nematodes). But, the most damages are the viruses that may destroy the entire harvest. Therefore, an important goal for pepper breeding is the development or selection of new varieties that shows resistance potentialities. The biotechnology can help the breeding techniques by: obtaining transgenic disease – resistant pepper cultivars, inducing mutations “in vitro” , obtainig virus-free plants using the in vitro specific methods etc. Any of these methods requires an efficient regeneration “in vitro” method which should allow the rapid and fidel multiplication of the valuable selected varieties.
Until now was reported: organogenesis from many explants (Agrawal eat al., 1989, Szasz et al., 1995), somatic embryogenesis from anther and immature zygotic embryos (Binzel et al., 1996) and even GMO (through Agrobacterium tumefaciens) – Kang et al., 1998. But until now there were no reports concerning the regeneration protocols for plant regeneration to Romanian pepper genotypes.
In our study the main goal was to determine the influence of the hormons over the regeneration of “in vitro” pepper plants. |
