The callus culture of leaves of Stevia rebaudiana was initiated and maintained on different strengths of Murashiage and Skoog (MS) medium supplemented with various phytohormones. Kinetin in combination with Naphthalene Acetic Acid (NAA) and 2,4-D showed better results in callus initiation whereas combined application of Benzyl adenine or 6-benzyl amino purine (BAP) and NAA showed most satisfactory results of callus maintenance in half strength of MS medium.
