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COMPARATIVE STUDY TO INDUCTION OF HAPLOID PLANTS BY “IN VITRO” ANTHER AND OVARIES CULTURE OF TOMATO (LYCOPERSICON ESCULENTUM MILL.)


MARIA PRISECARU, TINA OANA CRISTEA

Issue:

SCSB, Volume XII

Section:

Volume XII - Vegetal Biology

Abstract:

In order to induce experimental androgenesis and gynogenesis to tomatoes (Lycopersicon esculentum Mill.) there were used anthers with pollen in the uninucleated stage and unfertilised ovaries belonging to 15 genotypes.

Out of the 15 genotypes of tomatoes tested for their ability of forming haploids plants “in vitro”, two of them (L6 and L7) presented both androgenetic and gynogenetic aptitude, the L3 genotype produced plants from anthers, but L2, L5 and W1from ovaries.

An equal proportion of auxine and cytokinine (1:1) favors the induction of callus, but also it’s evolution to embryos and plants from anthers. The association of cytokinine with GA3 or only in the presence of cytokinine allows the callus evolution gynogenetically in embryos and plants. The rapport 2 to 1 between cytokinine and auxine determined a rizogenetic evolution of the formed meristematic centres. In order to develop the radicular system is not necessary to introduce phytohormones in the culture medium.

The preliminary cytogenetic study done on 15 gametoclones showed a high frequency of haploids (67%) compared with the regenerated diploid plants (33%) and the absence of other ploidy degrees.

Keywords:

Lycopersicon esculentum – anther and ovary culture – callus – haploids plants.

Code [ID]:

SCSB200712V12S02A0010 [0002489]


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