Abstract: | Micropropagation of H. perforatum, selected in experimental field in Piatra-Neamt with a view to optimising the regeneration processes (i.e caulogenesis, risogenesis), adjustment and transfer of fully adjusted plants in the experimental field. An average of 330.70 shoots were obtained at 35 flasks 14-18 days after the inoculation of callus on RM agar culture medium, supplemented with 1.0mg/l BAP and 0.5mg/l IAA or NAA, whereas on 0.5mg/l BAP, 1.0mg/l Kin and 0.5mg/l IAA supplemented medium only 136.10 shoots were obtained. In vitro risogenesis rate is of 87 - 98%, the best values (i.e., 98%) were obtained with MRI culture medium though the most vigorous shoots were obtained on MS hormone-free medium. Plant adjustment was observed in parallel, in sterile soil pots and under hydroponic system. In both cases they are covered. Under hydroponic system, restoring plant water equilibrium took 10 to 14 days whereas the adjustment rate obtained was of 89.4%. Some 57 adjusted plants were transplanted in the experimental field. |
