Origanum vulgare L. – oregano, is a perennial plant of 0.6 - 0.8 m, that belongs to the Lamiaceae family. Oregano is an important aromatic plant utilized both as culinary and medicinal plants. Tissue culture „in vitro” is a useful method for large scale production of pathogen-free plants. In this study in order to determine the best hormone variant that allows the obtaining of a large number of plants, apical shoots of young plants grown in controlled conditions were utilised. The explants were cultured in solid MS medium supplemented with different concentrations of kinetin – 1.0 – 2.0 mg/L and 1.0 - 2.0 mg/L BAP in combination with 0.1 – 0.5 mg/L NAA and IAA. Multiple shoots were obtained from the apical explants, the higher frequency (85%) formation of shoots was observed in the media variant that contained BAP in combination with NAA. Initially 1 or 2 buds developed, later up to 12 shoots of above 3 cm length were formed in node in two weeks. Shoots were multiplied by subculture on the same medium. The shoots rooted on the same media. The rooted plantlets were hardened and successfully established in pots at 85% success rate. The reported experimental dates represent viable methods of plant regenerations of Origanum vulgaris L. through shoot tip culture.
