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SOME CONSIDERATION REGARDING THE “IN VITRO” MORPHOGENETIC REACTION OF PELARGONIUM SPECIES


OANA CRISTEA, MARCELA FALTICEANU, MARIA PRISECARU

Issue:

SCSB, Volume XVI

Section:

Volume XVI - Vegetal Biology

Abstract:

Pelargonium is one of the world’s most important bedding and pot plants. Recent figures show that the annual sales in Europe and North America are worth in excess of US$ 700 million annually (Mithila et al., 2001). According to Cassells (1992) over 90% of the crop is vegetatively propagated. The main problem in tissue culture propagation of Pelargonium has been the high tendency to formation of vigorously growing callus with low organogenic potency and rapid senescence of cultures. Moreover, the significant differences in requirements to the medium composition (minerals, organic compounds and growth regulators) between Pelargonium cultivars have been observed. This makes difficult to develop a universal method of Pelargonium micropropagation; different approaches should be revised before the introduction in “in vitro” cultivation system of different valuable genotypes.

The paper presents the results of the investigations concerning the “in vitro” culture initiation of Pelargonium species, the behavior of the explants on different nutritive mediums, the obtaining of neoplantlets and their accommodation to aseptic conditions. Single node explants were inoculated on basal MS (Murashige and Skoog, 1962) medium containing 3% (w/v) sucrose, supplemented with different concentrations and combinations of 6-benzylaminopurine (BAP), kinetin (KN), indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA) and GA3 for direct plant regeneration. The best reaction - direct bud formation was observed on explants cultured on MS medium added with BAP. The combination of BAP and NAA increased the percentage of organogenesis and the development of the explants.

Keywords:

[n/a]

Code [ID]:

SCSB200916V16S01A0004 [0003189]


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