Abstract: | For the induction of âin vitroâ gynogenesis at wheat (Triticum aestivum L) the biologic material was represented by unfertilised ovaries excised from immature tillers belonging to six genotypes. A part of the ovaries placed in obscurity formed callus on the induction media N6 (Chu, 1978), vitamins MS or NB with 2,4 D 2 mg/l. The callus, placed in a regime of continuous light (1500 lx), on the culture media MS (Murashige-Skoog, 1962), with BAP and NAA (0,1 mg/l), Kinetin and NAA (0,5 mg/l), in absence of phytohormons or with GA3 0,1 mg/l, generated embryoids and plantlets, in case of three genotypes.
The frequency of embryogenic callus induction varies between 37 and 50%, and the regeneration one between 6 and 13%. The highest part of embryoids formed degenerated in different stages of evolution.
The cyto-histological study accomplished in the stained ovaries confirmed the presence of cellular activity in embryonic sacs which can imply the oosphere and/or synergids. From a total of 35 gynogenetic plants analised from cytogenetic point of view, on root meristematic metaphases, 21 were haploids, 13 diploid and one mixoploid. |
