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STUDIES ON THE IN VITRO BEHAVIOR OF THE OVARIES OF BRASSICA OLERACEA VAR. BOTRYTIS


DANIELA NICUŢĂ, GOGU GHIORGHIŢĂ

Issue:

SCSB, Volume XVIII

Section:

Volume XVIII - Vegetal Biology

Abstract:

The study of the in vitro behavior of broccoli ovaries was conducted using as biological material four genotypes, harvested from VRDS Bacau. The response of the ovaries inoculated on 8 nutrititional variants was characterized by two types of reaction: the generation of compact and brittle calli and the emergence of shoots by direct organogenesis. The gynogenetic callus emergence, its consistency, color and regenerative capacity depended on genotype, hormone formula of the nutritional media and size. O the BN and BDN media the ovaries of the BR-312-3 and BR-312-5 genotypes generated green brittle callus, characterized by high caulogenetic capacity. The emergence of shoots directly from the ovaries has been proven on the BDN and BN media, but the phenomenon had reduced intensity. The rooting of shoots was achieved on the MS medium without growth regulators. In vitro plants have been accommodated without problems in the hydroponic culture system.

Keywords:

ovary, Brassica, callus, sprouts.

Code [ID]:

SCSB201018V18S01A0016 [0003253]

Full paper:

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