Quick search
Go!

PRELIMINARY STUDIES ON THE INFLUENCE OF EXOGENOUS HORMONAL FACTORS ON THE „IN VITRO” ORGANOGENESIS OF BRASSICA OLERACEA L. VAR. CAPITATA


TINA OANA CRISTEA, MARIA PRISECARU, MARIA CĂLIN

Issue:

SCSB, Volume XVIII

Section:

Volume XVIII - Vegetal Biology

Abstract:

The implication of tissue cultures „in vitro” in the conservation of valuable genotypes of Brassica oleracea has a tremendous importance for the breeding activity of this species, due to its increased multiplication outturn, aspect associated with also with the assurance of a high degree of uniformity and conformity of the obtained descendencies.

Comparing with the classical methods, the tissue culture “in vitro” presents the following advantages: the process is not limited by the seasonal changes imposed by the succession of seasons, the control of growth regulators is assured, it prevents the manifestation of negative influences determined by the expression of genetic variability in descendencies, the genetic conservation of selected plants is assured for a longer period of time. An extremely important stage for the establishment of an efficient multiplication method “in vitro” is the determination of optimum conditions for the induction, support and development of regenerative processes of cultivated explants.

The factors that influence the capacity of regeneration of plants “in vitro” are numerous, but among them, the most important are the exogenous growth regulators.

Thus, for the determination of the best hormonal combinations, in the present study the authors tested 8 medium variants, that differ both from the point of view of quantity of hormons and the combinations between them.

The results obtained, indicated that the best morphogenetic reaction is obtained on variant with Zeatine as the main growth regulator, the results on this variant being superior comparing with the mediums with BAP.

Keywords:

PGR, zeatine, BAP, cabbage, vitro.

Code [ID]:

SCSB201018V18S01A0018 [0003255]

Full paper:

Download pdf


Copyright (c) 1995-2007 University of Bacu