Abstract: | In vitro ovary cultures were initiated at Brassica juncea Czern species in view of evincing their morphogenetic reaction, and also for a possible use of this technique as an alternative to provide haploid regenerants.
Our experiment started with the excision of the ovaries from the floral buttons. The ovaries were inoculated on several variants of nutritious media (enriched with growth regulators) to induce the development of gynogenetically derived structures. The basal medium was MURASHIGE –SKOOG, 1962 (MS), enriched with various concentrations and combinations of growth regulators. This type of phytoexplants provided two types of morphogenetic reaction. Therefore, depending on the combination of growth regulators within the initiation medium variants, some explants generated callus, and others provided off shoots directly from the ovaries on several medium variants. More than half of the inoculated ovaries displayed a morphogenetic reaction on most of the tested medium variants.
The main in vitro reaction was the production of rhysogenetic friable cream callus (which was highly proliferative on BN Ĺźi BB2 medium variants, followed by KN), and a caulogenetic compact green callus, highly proliferative on BA, BD, BN, KD Ĺźi KN variants. The nutritive medium variants B02 and B2 also provided shoots with floral stems. Some of the in vitro obtained offshoots were affected from chlorophyllian deficiency. |
