ASSESSMENT OF IN VITRO MORPHOGENETIC REACTION OF BRASSICA OLERACEA L. ANTHERS REGARDED AS GENOTYPE DEPENDENCY
CRISTEA OANA TINA 1, CALIN MARIA 1, BREZEANU CREOLA 1, BREZEANU PETRE MARIAN 1, PRISECARU MARIA 2 1. Vegetable Research and Development Station Bacau, Calea Barladului, No. 220, Bacau, Romania e-mail: tinaoana@yahoo.com 2. "Vasile Alecsandri" University of Bacau, Faculty of Science, Department of Biology, Marasesti Street, no. 157, Bacau, Romania e-mail: prisecaru_maria@yahoo.com
The aim of the present study was to assess the influence of donor genotype over the in vitro morphogenetic reaction of anthers cultivated on solid growth media. This is a primary step that needs to be assessed in any research that targets toward the establishment of a viable working protocol for the cultivation of anther in vitro and the obtaining of double haploid lines. During the experiment, we investigated the performance of 16 different genotypes (lines, varieties and hybrids). The seeds were provided by the Vegetable Research and Development Station Bacau, Romania.The results obtained in the present study showed that the ability of anthers for cultivation in vitro strongly depends on the genotype of the donor mother plants. There are genotypes that are totally recalcitrant for cultivation in vitro, while other showed an increased receptivity for regeneration of plants. The reaction of anthers was oriented toward the induction of direct organogenesis and embryogenesis or toward callusogenesis. Only in two of the studied genotypes (line BC145 and variety ZM 12) the anthers have shown no ability to cultivation in vitro.
