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THE PROCESS AND VALIDATION OF METHANOL DETERMINATION FROM BIOLOGICAL SAMPLES BY MEANS OF A GAS-CHROMATOGRAPHIC METHOD TOGETHER WITH MASS SPECTROMETRY


DOINA MOALES 1, ADRIAN FLORIN SPAC 1, MARIA PRISECARU 2, ELENA BUTNARU 1
1. University of Medicine and Pharmacy “Gr.T.Popa”, Faculty of Pharmacy, Universitatii street, no. 16, Iasi, Romania, Department of Toxicology, Department of Physical Chemistry e-mail: moldoina@yahoo.com
2. „Vasile Alecsandri” University of Bacau, Faculty of Science, Department of Biology, Marasesti Street, no. 157, Bacau, Romania e-mail: prisecaru_maria@yahoo.com

Issue:

SCSB, Number 2, Volume XX

Section:

Volume 20, No.2 - Animal Biology

Abstract:

In order to determine the methanol

concentration, the biological samples (blood,

tissues) were analyzed by an Agilent

Technologies 7890 gas chromatograph with an

Agilent Technologies 7683 Series automatic

injector equipped with a Zebron – Phenomenex,

ZB-WAX plus column (60 m x 0.25 mm, 0.25

µm), and Agilent Technologies 5975C inert MSD

detector; data acquisition was made by SIM

method. Data interpretation: Agilent

Technologies ChemStation software. The

temperature program begins from 50°C (constant

for 16 minutes), after that. the temperature

increase with 25°C/min up to 250°C, the mobile

phase (He) with a debit of 1 ml/min. The source

temperature is 230°C and the quadruple’s is 150

°C. In these conditions. the method is linear in the

5 – 20000 μg/mL range. the detection limit is

417.8 μg/mL, the quantification limits is 1266.1

μg/mL. There were determined: the system’s

precision (RSD = 0.8748%), method precision

(RSD = 4.6811%), intermediate precision (RSD =

4.1078 %); the accuracy was 98.0% in 93.2 –

102.8% range. Under these conditions, the

methanol concentration was determined from

biological samples, that is, blood and tissues.

Keywords:

methanol, blood, tissues, GC-MS.

Code [ID]:

SCSB20112002V20S01A0003 [0003720]


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