The experimentations were performed during
2011-2012 in Laboratory of Plant Tissue Culture
from Vegetable Research and Development Station
Bacau.
Regeneration, in all its aspects – callus, shoot
and embryo formation and rooting of newly formed
plantlets are key points on which relies the entire
concept of in vitro tissue culture.
Therefore, the main objective of the present
study was the assessment callus and shoot induction
in white cabbage anthers cultivated on media with
different concentration and combination of PGRs.
Anther-derived calluses, normally
accompanied by elongating small buds, are induced
in response to type of auxins added and subsequently
to combination with cytokinin, especially 6-
benzylaminopurine (BA).
The formation of callus structure was the
predominant morphogenetic reaction on media with
2,4 D, while NAA improved the embryogenic
competence of anthers.
The results of the present study also support
the idea of major impact involvement of PGRs in the
expression of cells totipotence. | 
