PLANT REGENERATION FROM ANTHERS CULTURE OF BRASSICA OLERACEA L.
MARIA PRISECARU 1, NICOLETA BĂDĂLUŢĂ 2, CRISTEA TINA OANA 3 1, 2 - University „Vasile Alecsandri” Bacau, Faculty of Biology, Marasesti Street, no. 157, Bacau, Romania
e-mail:prisecaru_maria@yahoo.com;nicoleta_dogaru@yahoo.com
3 - Vegetable Research and Development Station Bacau, Calea Barladului, No. 220, code 600388, Bacau, Romania
e-mail: tinaoana@yahoo.com
There was initiated an in vitro culture of immature anthers from Royal Delphy F1 hybrid and the inbred lines BCO 7-10, Z2 and Z2-12-2 of cabbage (Brassica oleracea var. capitata). For callus induction, regeneration and risogenesis, the MS (1962) medium was used with various concentrations of hormones. The incubation of anther cultures was done in the dark for 6 days at a temperature of 35 C; after this period, they were transferred to 23 C for 14 days. The induction efficiency of embryonic callus and shoots was good, especially for Royal Delphy and Z2-12 genotypes. Of phytohormones, BAP in concentration of 2.1 ml/l can induce not only organogenesis, but also embryogenesis in callus cultures when associated with NAA in concentration of 0.5 ml /l. The accommodation of plants was achieved by their passage through a hydroponic system for 14 days. Accommodated plants were planted in pots with a soil/perlite 1/1 mixture, then in greenhouse and in experimental fields.
