PHYLOGENETIC RELATIONSHIPS WITHIN CARASSIUS GENERA
(FORTUNA LAKE - DANUBE DELTA) USING MITOCHONDRIAL DNA
MARKERS
IONESCU MIHAELA-LILIANA 1, GHIORGHIŢĂ I. GOGU 2 1. ”Alexandru Ioan Cuza” University of Iasi, Adress: ”Al. I.
Cuza” University of Iasi, Carol I Bvd. 20A, RO-
700505, E-mail:
mihaela.ionescu84@yahoo.com
2. Academy of Romanian Scientists, Piatra Neamt Branch
e-mail: g.ghiorghita43@yahoo.com
In this experiment we investigated the genetic diversity within Carassius genera, studying individuals from Fortuna Lake (Danube Delta), using the sequencing of cytochrome b (Cyt b), mitochondrial control region (D-loop) and cytochrome c oxidase subunit I ((COX I (CO I) as markers. The variation of the nucleotide sequence for the three genes were used to study the mtDNA divergence for Carassius genera individuals and to examine the phylogenetic relationships within analyzed individuals.Based on the alignment of the three mitochondrial genes were identified the new haplotypes, for Cyt b and D-loop seven new haplotypes, while for COX I were recorded eight new haplotypes.
Regarding the frequence of the new haplotypes within analysed population we observed that, for Cyt b the haplotype C02DL is the most frequent (25%), for D-loop all haplotypes have the same frequence 14,29% and for COX I the general haplotype is C04D (73,08%). The analysis of phylogenetic trees showed that all the investigated individuals originate from the same common ancestral node.
