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MICROPROPAGATION OF HYPERICUM PERFORATUM L. USING AGNO3 TO ENHANCE SHOOT REGENERATION


TINA OANA CRISTEA 1, MARIA PRISECARU 2, SILVIA AMBARUS 1, MARIA CALIN 1, CREOLA BREZEANU 1, MARIAN BREZEANU 1, GEORGE FLORIN ŞOVA 1
1. Vegetable Research and Development Station Bacau, Calea Barladului, No. 220, Bacau, code: 600388, e-mail: tinaoana@yahoo.com
2. „Vasile Alecsandri”, University of Bacau, Faculty of Science, Department of Biology, Ecology and Environmental Protection, Marasesti Street, no. 157, Bacau, Romania, e-mail: prisecaru_maria@yahoo.com

Issue:

SCSB, Number 1, Volume XXIV

Section:

Volume 24, No. 1

Abstract:

The present study was undertaken in the Laboratory of Plant Cell ad Tissue Culture from Vegetable Research and Development Station Bacau, Romania. The purpose of the study was the enhancement of plant regeneration efficiency at Hypericum perforatum L. cultivated in vitro. As well known silver ions, in the form of nitrate play an important role in promoting the somatic embryogenesis and organogenesis, which led its wide spread use, is in different plant tissue culture in vitro. Thus, the investigations were focused toward the establishment of its role in sustaining the plant regeneration in vitro and the achievement of a rapid multiplication procedure at Hypericum perforatum (a valuable herbaceous medicinal plant). The explants excised from healthy mother plants were cultured on Murashige-Skoog, 1962 medium with BAP – 8.9 µM and IAA – 1.1 µM supplemented with different concentration of AgNO3 (5 µM, 10 µM, 15 µM, 25 µM, 30 µM). In general, NAA alone promoted root induction at all the regenerated shoots. Rooted shoots were successfully re-established in soil under controlled conditions

Keywords:

organogenesis, embryogenesis, regeneration, plants.

Code [ID]:

SCSB201501V24S01A0009 [0004391]

Full paper:

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