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USE OF IN VITRO CULTURES TO OBTAIN PLANT MATERIAL AT THE SPECIES VERONICA OFFICINALIS L., NEEDED TO MAKE BIOPRODUCTS


DANIELA NICUŢĂ
“Vasile Alecsandri” University of Bacău, Department of Biology, Ecology and Environmental Protection, Marasesti Street, 157, Bacau, e-mail: danan@ub.ro

Issue:

SCSB, Number 1, Volume XXV

Section:

Volume 25, No. 1

Abstract:

Veronica officinalis L., native to Europe and Western Asia, commonly known as speedwell, gypsyweed or Pauls’ betony etc., is used as a medicinal plant because of its content of tannins, bitter substances, volatile oil, saponins, flavones and glycosides. Because of these bioactive compounds is recommended for its actions and effects both internally – stomachic, cholagogue, digestive tonic, gentle expectorant, anti-catarrhal, diuretic, astringent, hemostatic, cleansing, anti-inflammatory –, and externally – healing skin wounds and scars, astringent, hemostatic. Among the many advantages provided by the in vitro culture technology at plants, there are: the possibility to preserve the valuable qualities of a genotype by cloning it, as well as the induction of genetic variability in order to obtain genotypes (somaclones) that are more valuable in terms of the content of bioactive compounds. To initiate in vitro cultures of Veronica officinalis L. there were used young shoots harvested from a population at wild flora. As a basic nutrient medium there was used Murashige-Skoog (1962), enriched with several combinations and amounts of growth regulators. Our observations led to the following conclusions: on most nutrient media supplemented with growth regulators, the node and apex explants had a good reaction, generating new shoots and roots. The aspect and number of shoots and the rizogenetic intensity varied depending on the type and concentration of the growth regulators present in the nutrient medium. A proper morphogenetic response was noticed for the explants inoculated on the medium variants of MS enriched with 1mg/l BAP (benzylaminopurine) + 0.1 mg/l IAA (indole-3-acetic acid), 1mg/l BAP (benzylaminopurine) + 0.5 mg/l ANA (naphtyl-acetic acid) and 1mg/l Kin (kinetine) + 0.5 mg/l ANA (naphtyl-acetic acid). The calusogenetic reaction at the inoculated explants was highlighted sporadically, with low yields on some hormonal formulas. The acclimatisation of the vitroplants to the ex vitro environment encountered no problems, and took place in a hydroponic system.

Keywords:

Veronica officinalis, in vitro culture, caulogenesis, micropropagation, bioproducts.

Code [ID]:

SCSB201601V25S01A0017 [0004581]

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