STUDIES ON THE MORPHOGENETIC REACTION OF ORIGANUM VULGARE L. EXPLANTS IN VITRO CULTIVATION
DANIELA NICUŢĂ, IRINA LAZAR “Vasile Alecsandri” University of Bacau, Department Biology, Ecology and Environmental Protection, e-mail: dana_nicuta@yahoo.com
The species Origanum vulgare has been known since Antiquity as a plant with a high content of bioactive compounds, especially in plant leaves and flowers. The biochemical analysis of the vegetative parts of oregano plants revealed the presence of essential oregano oil. It contains mainly two phenols, carvacrol and thymol, of great economic importance. Due to the chemical composition of essential oil, oregano plants are used in the pharmaceutical, cosmetic and food industries. Our research was aimed at in vitro cultivation of Origanum vulgare explants. Apexes and nodes were grown on several nutritional variants supplemented with BAP, IAA and NAA, at different concentrations. The main morphogenetic reaction was caulogenetic reaction. At the base of the shells formed from the explants, roots were formed. The shoots obtained on all tested variants generally showed good and very good growth, depending on the phytohormonal combination present in the nutritive medium. The best shoots yield was found on the media supplemented with BAP – 1ml/l and IAA – 0,5 ml/l.
