The research presented in this paper focused on the cariotypic characterization of Echinacea angustifolia D.C. existing in the collection from S.C.D.L. Bacau. Genetic characterization is important for species identification and hybrid population analysis. Genetic determinations include chromosomal counting and karyotype determination, mitotic indexing, and mitotic anomaly analysis. The biological material used in the present cytogenetic study is represented by the radicular meristems of the plantlets resulting from the germination of the seeds of Echinacea angustifolia D.C. The preparation of biological material for cytogenetic studies was carried out according to the data presented by the literature. Thus, two working methods were used to determine the best coloring method, namely the Schiff Reagent staining method and Carr's Reagent staining method. Measurement of chromosomes was made by correlating the measurements made using the microscope micrometer and the measurements made in Adobe Fotoshop 5. The results obtained showed that the chromosome sizes varied between 4.12 and 5.83 ÎŒm. Four pairs of chromosomes with centromers in the median area and two pairs with centromers in the sub-median area were identified. Of the remaining five pairs of chromosomes, four have centromers in the sub-terminal area, and the tenth pair has the centromer in the terminal region.
