RESPONSE SURFACE METHODOLOGY USED FOR THE OPTIMIZATION OF THE FLAVONOID EXTRACT OBTAINING TECHNOLOGICAL PROCESS
FLORENTINA N. RONCEA 1*, RADU G. CAZACINCU 2 (1)OVIDIUS UNIVERSITY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACEUTICAL TECHNOLOGY, CONSTANTA, ROMANIA (2)OVIDIUS UNIVERSITY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACEUTICAL INDUSTRY, CONSTANTA, ROMANIA
(*)CORRESPONDING AUTHOR: FLORENTINARONC
The objective of this work was to use Box – Wilson experimental plan to establish the relation between two dependent variables (Y1 (total polyphenols) and Y2 (antioxidant capacity mM TROLOX/g extract)) and independent variables X1 (vegetal product/solvent ratio), and X2 (hydrolysis time) in order to obtain the optimal formula of the technological process using the response surface methodology (RSM). The optimal formula represents one or two formula (solutions of the system) that analyzed the correspondence with the predicted values. To calculate the coefficients for the response equation we used a software running under Excel. For the optimal formula of the technological process we used Matlab 6.5 version (for plots) and for superimposing the Adobe Photosmart – Acrobat Reader software. Statistics ("t" Student test) were generated through the Excel software. The estimation error values prove the validity of the mathematical method used. The vegetal product/solvent ratio (mass/volume) is 9.25, the hydrolysis time is 18 hours. These solutions correspond to the optimal value and are statistically validated. The software used is running under Windows and could be used for other formulations as well.
