Abstract: | Utilizing the “in vitro” culture of unfertilized ovaries we succeeded on obtaining plants with gynogenetic origin via callus, at the potato variety Desirée (Solanum tuberosum L.). For the induction of gynogenesis the best hormonal combination was: BAP (0,3 mg/l), NAA (0,1 mg/l) and 2,4 D (0,05 mg/l). The basic media utilized in our experiments was MS (1962), supplemented with active charcoal (0,5 mg/l). The differentiation media with BAP (1-2 mg/l) associated with NAA or IAA (0,5 mg/l) generated embryos and plants.
The association of BAP or zeatin with GA3 (0,5 mg/l) determined the formation of a large number of micro-tubers both on plants and in the interior of media. The micro-tubers formation was stimulated also by a larger quantity of sucrose (8 %). The micro-tubers and fragments of plantlets represented a good material for “in vitro” micropropagation. The plants obtained from the gynogenetic callsu were 56% diploid, 28% haploid, 3 plants aneuploid and one mixoploid. |
