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CYTOPHOTOMETRIC STUDY OVER "IN VITRO" PLANTS REGENERATED FROM ANTHERS AND OVARIES AT BRASSICA OLERACEA L AND B. JUNCEA CZERN SPECIES


DANIELA NICUŢĂ, JOAO LOUREIRO, GOGU GHIORGHIŢĂ, GHICA MIHU

Issue:

SCSB, Volume XVI

Section:

Volume XVI - Vegetal Biology

Abstract:

Flow cytometry tests were ran in order to establish the ploidy level for 24 regenerants from anthers and ovaries of two Brassica species.

Most of the B. oleracea individuals were myxoploids, having diploid, tetraploid and a small number of octoploid cells.

Four regenerants were the exception: three of them had a DNA amount specific to diploid level and the fourth proved to be a tetraploid.

Three regenerants of B. juncea (tetraploid species) were tested, two of them provided by ovaries and one by anthers, all of them being dihaploids. Considering that brown mustard has a better response to experimental haploidy than cabbage, more detailed investigations are necessary in order to control this process.

Keywords:

[n/a]

Code [ID]:

SCSB200916V16S01A0014 [0003199]


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