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A COMPARATIVE STUDY REGARDING SOME PHENOTYPIC AND GENETIC FEATURES OF IN VITRO MICROPROPAGATED AND SEED-BORNE TOMATO (LYCOPERSICON ESCULENTUM MILL) PLANTS


TINA OANA CRISTEA, MARIA PRISECARU, MARIA CĂLIN

Issue:

SCSB, Volume XVIII

Section:

Volume XVIII - Vegetal Biology

Abstract:

Tomato (Lycopersicon esculentum Mill.) is a major vegetable crop that has a tremendous popularity, being cultivated in almost every country of the world either for fresh market or for processing.

Due to its economic worldwide present and future potential of improving the crop through molecular techniques, improvement in the efficiency of regeneration is expected to have a positive impact on transformation results.

The present study aimed at the accomplishment of a comparative study regarding the main agronomic features of tomatoes plants regenerated in vitro and seed-grown tomato plants. Morphological, fruit yield and genetic features were compared between the seed-grown and tissue cultured plants of two genotypes of tomato (Lycopersicon esculentum Mill) L24S and L27S in greenhouse conditions at V.R.D.S. Bacau. The results obtained in the present study showed that there were no phenotypic or genetic abnormalities at tissue-cultured plants compared with the seed-borne plants.

This study proves that genetic fidelity of tissue cultured plants can be maintained if appropriate plant growth regulators are used with fewer member of subcultures in the multiplication medium.

Keywords:

genetic, stability, multiplication, vitro.

Code [ID]:

SCSB201018V18S01A0011 [0003248]

Full paper:

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