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RESEARCH ON THE INFLUENCE OF CARBON SOURCES ON THE DYNAMICS OF CATALASE ACTIVITY IN FUNGUS MONILINIA LAXA (ADERH.&RUHL.) HONEY PARASITIC ON PLUM


TUTU ELENA, CIORNEA ELENA, COJOCARU SABINA IOANA
"Alexandru Ioan Cuza" University of Iasi, Faculty of Biology, Carol I Bvd., 20 A, Iasi, Romania
e-mail: elenatutu2010@yahoo.com
e-mail: ciornea@uaic.ro
e-mail: sabina_18ro@yahoo.com

Issue:

SCSB, Number 1, Volume XX

Section:

Volume 20, No. 1 - Vegetal Biology

Abstract:

It is well known that oxidoreductases constitute group of enzymes which are strictly necessary for the microorganisms being involved in the decomposition reactions of hydrogen peroxide formed during the oxidation processes catalyzed by flavin-enzyme. In addition to the detoxification function, the catalyses constitute also a terminal electronic flow regulator from the chain respiratory processes, and it is also a „trap” for free radicals that appear from radiolysis. This paper systematizes a series of experimental results regarding the influence of different carbon sources on the dynamic activity of catalase in Monilinia laxa (Aderh.&Ruhl.) Honey species – a parasite fungus on stone fruits from different species of Rosaceae. The activity of catalase - the bicomponent oxidoreductase involved in oxidative stress was determined using the titration method, both in the fungus mycelium and in liquid culture. For each sample, there were performed 3 parallel determinations and the results were statistically processed by calculating on the basis of the mean values and standard deviation, with a probability of 95%, the superior and the inferior limits of confidence intervals. Experiments consisting of consecutive measurements made at 7 and 14 days after the inoculation of culture medium marked out the fact that the enzyme activity was significantly influenced both by the culture age and the type of carbohydrate present in the fungus culture medium.

Keywords:

Monilinia laxa (Aderh & Ruhl ) Honey, plum, oxidative stress, catalase, carbohydrates.

Code [ID]:

SCSB20112001V20S01A0023 [0003700]


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