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THE STUDY OF COMPOSITION IN FREE AMINOACIDS AT HAPLOID GAMETOCLONES WITH GYNOGENETIC ORIGIN AT TRITICUM AESTIVUM L., BETA VULGARIS L., HELIANTHUS ANNUUS L. AND SOLANUM TUBEROSUM L.


MARIA PRISECARU 1, TINA OANA CRISTEA 2, MARIA CALIN 2
1. „Vasile Alecsandri” University of Bacau, University „Vasile Alecsandri” of Bacau, Faculty of Science, Department of Biology, Ecology and Environment Protection, Marasesti Street, no. 157, Romania e-mail: prisecaru_maria@yahoo.com
2. Vegetable Research and Development Station, Calea Barladului, Street no. 220, Bacau, Romania e-mail: tinaoana@yahoo.com

Issue:

SCSB, Number 1, Volume XXI

Section:

Volume 21, No. 1 - Vegetal Biology

Abstract:

The aminoacids that are present in the

haploid plants of 10-12 cm length obtained by

gynogenesis „in vitro” via the culture of nonfertilized ovaries and ovules have been identified

and quantitatively measured in wheat (Triticum

aestivum L.), sugarbeet (Beta vulgaris L.),

sunflower (Helianthus annuus L.) and potato

(Solanum tuberosum L.) by using the

chromatographic method upon thin larger and

UV-VIS spectrophotometry.

Diploid plants of the same length where

used for control. They were obtained by

germination of the donor plants seeds from where

ovaries and ovules for cultivation in vitro were

sampled. Thus, it is found that in the haploids

obtained in vitro, for all studied species, the

values of aminoacids are in general clearly low,

as compared to the control diploid plants.

The haploid plants chromatograms show

also the lack of some aminoacids identified in the

control diploid plants.

It is interesting to note that in some

haploids new aminoacids appear, but they are not

identified in the 2n plants (phenylalanine, in the

potato haploid), or in almost equal quantitative

values, or higher that the 2n donor plant value (

this in the case of methionine in wheat haploid

and histidine in sunflower haploid).

Keywords:

in vitro gynogenesis, haploid plants, free aminoacids, chromatographic and spectrophotometric analisis, qualitative and quantitative determination.

Code [ID]:

SCSB20122101V21S01A0009 [0003751]


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