ASPECTS REGARDING THE IN VITRO MULTIPLICATION OF THE
RUBUS HIRTUS L. SPECIES
NICUŢĂ DANIELA 1, ROTILĂ GABRIELA 2, CIOBANU ŞTEFAN 3 1,2,3 “Vasile Alecsandri” University of Bacău, Faculty of Sciences, Department of Biology, Ecology and Environmental Protection,
e-mail: dana_nicuta@yahoo.com
The blackberry perennial shrub belongs to the Rosaceae family. It is among the oldest medicinal plants with Eastern origins, the proof of its medicinal use dates from Hippocrates times, the 4th century B.C. Its berries are appreciated for their taste and mostly for their high amounts of vitamins (A and C); the leaves have a medicinal utility, due to their disinfectant, healing, antihemorrhagic properties, and for hormonal regulation as well. Due to its economic importance, the purpose of our research was to highlight the in vitro reaction of this species by observations on the morphogenetic response of a series of explants on various nutritive variants. At the same time, we aimed to identify a profitable technology to micropropagate blackberry. In view of initiating the in vitro cultures, uninodal and apical explants were used, harvested from young shrubs, disinfested with HgCl2 1‰ and chloramine-T 5%. After the removal of disinfesting solutions (by rinsing in distilled water), the explants were inoculated on various medium variants of basal MS (Murashige –Skoog,1962) and WPM (Woody Plant, Lloyd and Mc Cown, 1980) medium, enriched with several combinations and amounts of growth regulators. Our observations led to the following conclusions: a proper morphogenetic response was noticed for the explants inoculated on the medium variants of MS enriched with 1mg/l BAP(benzylaminopurine) + 1 mg/l Kinetin, and WPM enriched with 2 mg/l Zeatin + 1 mg/l BAP; 3 mg/l Zeatin + 0.5 mg/l IBA (indole-3-butyric acid) - in order to micropropagate the shoots; the presence of ANA (naphtyl-acetic acid – 1.5 mg/l) within the WPM medium induced the development of a strong radicular system. The acclimatisation of the vitroplants to the ex vitro environment encountered no problems, and took place in a hydroponic system. About 2 weeks after the acclimatisation, the new plants were transfered into soil pots.
