Quick search
Go!

CATECHOLAMINES AND RELATED COMPOUNDS SEPARATION ON A MIXED MODE COLUMN
SEPARATION DE CATECHOLAMINES ET MOLECULES APPARENTEES SUR UNE COLONNE EN MODE MIXTE


RALUCA-IOANA TAMPU 1*, OANA-IRINA PATRICIU 1, CLAIRE ELFAKIR 2
1. Université ”Vasile Alecsandri” de Bacau, Faculté d’Ingénierie, Département de Génie Chimique et Alimentaire, 157, Calea Marasesti, 600115, Bacau, Roumanie
2. Université d’Orléans, CNRS, ICOA, UMR 7311, Institut de Chimie Organique et Analytique, rue de Chartres, BP 6759, F-45067 Orléans, France
*Corresponding author: tampu.raluca@ub.ro

Issue:

SCSCC6, Volume XV, No. 2

Section:

Volume XV, No. 2 (2014)

Abstract:

The present paper presents the evaluation of a mixed mode column (reversed phase and cation exchange) performance for the separation of 12 catecholamines, indolamines and their precursors and metabolites. The influence of different parameters, like organic modifier nature and percentage, salts nature and percentage, on the compounds separation was investigated. Good separation of the 12 selected compounds was obtained in the isocratic mode using a mobile phase composed of 90 % methanol and 10% ammonium acetate 20 mM, however the analysis time was excessively long (80 minutes). Thus an elution gradient was optimized and it reduced the analysis time to less than half (35 minutes).

Keywords:

catecholamines, indolamines, mixed mode column, reversed phase liquid chromatography.

Code [ID]:

CSCC6201402V02S01A0003 [0004142]

DOI:

Full paper:

Download pdf


Copyright (c) 1995-2007 University of Bacău