UV/VIS SPECTROMETER DETERMINATION OF CAFFEINE IN GREEN COFFEE BEANS FROM HARARGHE, ETHIOPIA,
USING BEER-LAMBERT’S LAW AND INTEGRATED ABSORPTION COEFFICIENT TECHNIQUES
EPHREM G. DEMISSIE 1*, GIRMA W. WOYESSA 1, ARAYASELASSIE ABEBE 2 1. Haramaya University, College of Natural and Computational Science, Department of Chemistry, P.O. Box 138, Dire Dawa, Ethiopia
2. Haramaya University, College of Agriculture, School of Animal and range Science, P.O. Box 138, Dire Dawa, Ethiopia
*Corresponding author: ephrem1977@gmail.com
A total of fifteen samples of green coffee (Coffea arabica L.) beans from the major producing region of Hararghe Ethiopia were studied using UV-Vis spectrometer measurement caffeine quantitative analysis from coffee beans. The number density of caffeine in green coffee beans has been reported using Beer-Lambert’s law and integrating absorption coefficient technique. Our results obtained using integrated absorption and Beer-Lambert’s law has a good agreement and we observed a maximum difference of 10.4 %. Based on their low caffeine concentrations among the samples collected were found in Jarso coffee. Coffee beans from the Harar Aboker were characterized by higher concentrations of caffeine. The determined concentration for caffeine in coffee beans (% w/w) ranged 0.601 % to 0.903 %. The concentrations of the caffeine varied significantly, depending on the geographical origin of the beans. The concentrations of caffeine in coffee collected from in Hararghe region were noticeably lower than their counterpart (1.0 - 1.2 %) grows in the other parts of Ethiopia.
