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EXTRACELLULAR LIPASE PRODUCTION BY TRICHODERMA HARZIANUM ISOLATED FROM OIL CONTAMINATED SOIL


ALIMA RIHANI *, BOUDJEMA SOUMATI
University of Badji Mokhtar (UBMA), Faculty of Sciences, Department of Biochemistry, 23000, Annaba, Algeria
*Corresponding author: alimaprof@ymail.com

Issue:

SCSCC6, Volume XX, No. 1

Section:

Volume 20, No. 1 (2019)

Abstract:

The aim of this study was to investigate the ability of the filamentous fungus Trichoderma harzianum to secrete lipolytic activity and examined the influence of carbon on lipase production. A quantitative analysis of lipase activity was performed by the titration method using olive oil as a substrate. The isolated strain was cultivated in shaking flasks containing basal media and supplemented with olive oil 1 % (v / v) as lipid source. The effect of other carbon sources added to basal medium as lauric acid ester (Tween 80) and glucose was tested to improve enzyme production. Maximum biomass was produced at a concentration of (1906 mg∙mL-1 ± 4.58) and (622.5 mg∙mL-1 ± 9.19) in mineral medium supplemented with glucose (2 %) and Tween 80 respectively. However, the lipase activity was maximal for olive oil (1.58 IU∙mL-1 min-1 ± 0.11). The result obtained in this study indicated that olive oil proved to be the best inducer and stimulated lipase production. Glucose and Tween 80 supplied as additional carbon sources have not enhanced the lipase production, whereas they only increased the biomass of T. harzianum.

Keywords:

fungal lipase, glucose, lipolytic-producing fungi, olive oil, screening, Trichoderma harzianum, Tween 80.

Code [ID]:

CSCC6201901V01S01A0002 [0004835]

Note:

Full paper:

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