DETERMINATION OF CATECHOLAMINES AND RELATED MOLECULES IN BRAIN EXTRACT USING A HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY MASS SPECTROMETRY METHOD
RALUCA-IOANA TAMPU 1, 2*, ADRIANA FINARU 1, CLAIRE ELFAKIR 2 1. “Vasile Alecsandri” University of Bacau, Calea Marasesti, 157, Bacau-600115, Romania
2. Institute of Organic and Analytical Chemistry CNRS UMR 7311, University of Orléans, Chartres, BP 6759, 45067 Orléans, France
*Corresponding author: tampu.raluca@ub.ro
The present paper presents a hydrophilic interaction chromatography (HILIC) coupled to tandem mass spectrometry method for the analysis of 11 catecholamines, precursors and metabolites in a sheep brain extract. The LODs were between 0.5 ng∙mL-1 (3-MT) and 250 ng∙mL-1 (DOPAC). The method accuracy has been evaluated in brain extract by recovery studies with samples spiked at a concentration of 100 ng∙mL-1. For the spiked brain extract, the mean recovery values ranged from 81 % to 101 %, with precision < 8 %.
