Issue:

SCSCC6, Volume XXI, No. 1

Section:

Volume 21, No. 1 (2020)

Abstract:

The present paper presents a hydrophilic interaction chromatography (HILIC) coupled to tandem mass spectrometry method for the analysis of 11 catecholamines, precursors and metabolites in a sheep brain extract. The LODs were between 0.5 ng∙mL-1 (3-MT) and 250 ng∙mL-1 (DOPAC). The method accuracy has been evaluated in brain extract by recovery studies with samples spiked at a concentration of 100 ng∙mL-1. For the spiked brain extract, the mean recovery values ranged from 81 % to 101 %, with precision < 8 %.

Keywords:

HILIC, neurotransmitters, sheep brain extract, tandem mass spectrometry, TSK gel Amide 80.

Code [ID]:

CSCC6202001V01S01A0006 [0005094]

Note:

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