Cellular and intracellular structures of biological cells can be disturbed by exposure to nanosecond pulses electric field (nsPEF). The exposure system comprises two main parts: the pulses generator and the delivery device that containing biological medium. In this paper, we propose and characterize an open Transverse ElectroMagnetic (TEM) cell to expose a Petri dish filled with a biological suspension to nanosecond high voltage pulsed electric fields. Measurements of the reflection (S11) and transmission (S21) parameters show that empty TEM cell has a bandwidth of 2.9 GHz, whereas when the Petri dish is filled bandwidth is 1.7 GHz. TEM cell is well suited to deliver ultra-short pulsed electric fields with 1 ns duration.