Our study aims to identify vibrational differences associated with the cell cycle
by multiplex CARS micro spectroscopy in the CH zone. For this purpose, a human cell line
HEK293 is used and blocked either in interphase or prophase and then analysed by CARS
mapping on the vibration modes associated with proteins and lipids. We have shown that
using the vibrational mode of proteins, we can characterize the state of chromatin
condensation, moving from a low condensed state (interphase) to hypercondensed (mitosis).
This hypercondensation is ensured by the binding of condensins to chromatin, proteins that
are associated with chromatin to promote compaction. | 
