The interest of multiphoton microscopy (MPM) is now well established in life
sciences, especially thanks to its multispectral aspect. However, this property is limited by
commercial laser systems currently involved in MPM, presenting a short spectral
bandwidth. At best, four constituents can be imaged simultaneously. Here, we present an
original spectral filtering system coupled with a large bandwidth laser system. The
adaptation of this system to MPM in life sciences is demonstrated of target samples from
plant or animal origin. |