We had tried to establish the number of chromosomes and the caryotype for two species of amphibians – Rana esculenta and Rana ridibunda from Ciric Lake (Iasi).
The biological material was represented by individuals from these two species Rana ridibunda and Rana esculenta that provenance from Ciric Lake.
Each individual was injected intraperitoneal with 0,5% colchicin solution. After 2 hours, spleen and gonads were extracted and hypotonized with sodium citrate 0,8% for 30 minutes, at the room temperature. We used the dried air method for making samples. After 24-48 hour of ice keeping, all slides were dried at the room temperature and colored with May-Grunwald-Giemsa colorant 4%. All slides were analyzed at 40X objective and pictures were made with 100x immersion objective. | 
