Abstract: | One of the very important steps in a professional characterization of mutants must be the monitoring of DNA content, which can offer valuable information on mutagenesis effects on nucleic acids level (Cîmpeanu et al., 1996).
Proteins phosphorylation by kinases and phosphatase represent a very important post-transductional mechanism, in eukaryotic cells, in biological processes control (metabolic pathway, signals transmission, gene expression and cell cycle differentiation) (Yanagida et al., 1992). On S. pombe, acid phosphatase, another subject of our investigation, represents a key enzyme involved in cell surface modulation and cellular differentiation during cell cycle, especially in sexual process initiation (Schwaninger et al., 1990). Schweingruber and Schweingruber (1982) show that enzyme activity and its level is repressed on rich media (with abundant phosphate source) and derepressed on minimal media, or media used for induction of sexual cycle. It’s obvious that the investigations on acid phosphatase level may offer important information on sexual cell cycle on normal and mutant strains previously induced P1 – P12 (Pricop et al., 1996). |
