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PRELIMINARY STUDIES REGARDING CELL DENSITY AND TEMPORAL DYNAMICS OF BACTERIAL BIOFILMS FORMED AT LIQUID - SEDIMENT INTEFACE USING BRIGHT FIELD AND EPIFLUORESCENCE MICROSCOPY


AURELIA MANUELA MOLDOVEANU

Issue:

SCSB, Volume XIX

Section:

Volume XIX - Animal Biology

Abstract:

The biofilms represent communities of prokaryotes and eukaryotes that are formed in different types of hydrophile and hydrophobe surfaces determining the occurrence of microfouling, biocorrosion and the reduction of materials efficiency.

The generation of biofilms was accomplished based on a comparative study using seawater as culture medium and glass slides as artificial support for the adherent cells.

Only three days after the immersion of the artificial substrate, a dense layer of cells is formed on the hydrophile surface of glass and 15-18 days.

later, depending on the thickness of the biofilm, detachment and dispersion phenomena occur.

The formation of biofilms in static conditions occurs more quickly from density of 11∙102 cel/mm2 to 74∙102 cel/mm2 for the sea water and sediment area separate by the interface.

This information was obtained nondestructively, quasisimultaneously, and in real time, thereby permitting the verification of time-dependent relationships between the biofilms population structure, distribution, and interfacial chemistry. The approach offers opportunities to examine these relationships on a variety of substrata in the presence of a bulk aqueous phase under controlled hydrodynamic conditions.

Keywords:

biofilms, slides, static condition, hydrophile, buried slide, microbial capture.

Code [ID]:

SCSB201019V19S01A0012 [0003272]

Full paper:

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