THE INFLUENCE OF SOME THYAZYNIC AND PHENAZYNIC COMPOUNDS ON THE ACTIVITY OF SOME ENZYMES AT PHASEOLUS VULGARIS AND TRITICUM ESTIVUM IN THE GERMINATION PERIOD
DUMITRU GABRIELA 1, CIORNEA ELENA 1, VASILE SILVIA 2 1. "Alexandru Ioan Cuza" University of Iasi, Carol I Bvd., 20 A, Faculty of Biology, Laboratory of Biochemistry and Molecular Biology, Romania e-mail:gabriela.dumitru@uaic.ro e-mail: ciornea@uaic.ro 2. Comprehensive School Stiubei, Buzau County e-mail: sylvika_20@yahoo.com
The researches aimed at the analysis of the degree influence of two dyestuffs, namely neutral red and methylen blue â as derivates with phenazynical and, respectively, thyazynical structure, on to some physiological aspects of plants (the increasing degree of seedlings and the germination degree of the seeds) and on the activity of some enzymes with major role in the germination process of the wheat (Triticum aestivum) and, respectively, of the bean (Phaseolus vulgaris). Thus, the enzymatic activity was determined along 72 hours of germination, appreciating the influence of the chemical analyzed compounds, used in different concentrations (10^-1 M, 10^-3 M, 10^-5 M), on the mobilization degree of specifically substratum of the enzymes taken into the study. The analysis of the obtained results underlines the fact that the two dyestuffs used evince a striking action from physiological point of view, just as in what concerns the enzymatic activity, with variations more or less ample depending on the used concentration.
