STUDIES ON THE DYNAMICS OF EXTRACELLULAR AND ENDOCELLULAR PEROXIDASE ACTIVITY OF THE FUNGUS MONILINIA LAXA (ADERH.&RUHL.) HONEY PARASITE ON PLUM TREE
TUTU ELENA, CIORNEA ELENA, COJOCARU SABINA IOANA University "Alexandru Ioan Cuza" Iasi, Faculty of Biology, Carol I Bvd., 20 A, Iasi, Romania e-mail: elenatutu2010@yahoo.com e-mail: ciornea@uaic.ro e-mail: sabina_18ro@yahoo.com
The experimental research attempted to elucidate some aspects regarding the influence of carbohydrates on the dynamics of peroxidase activity, the enzyme which plays the most important role in the cellular process of oxidoreduction, correlated with the respiration process, at the Monilinia laxa (Aderh.& Ruhl.) Honey species. Peroxidase activity was determined using o-dianisidine method, for each sample we made 3 parallel determinations in both the fungus mycelium and in the liquid culture at 7 and 14 days old inoculations. Subsequently, the data obtained was statistically processed by calculating the average, error and standard deviation, variation and precision coefficient of the mean and confidence interval limits. The experimental results showed the existence of some significant differences from one sample to another, endocellular and extracellular peroxidase activity being strongly influenced by the type of carbon source used in the culture medium and by the age of the culture.