COMPARATIVE RESEARCHES ON THE HYDROLYSIS DEGREE OF THE RESERVE SUBSTANCES UNDER THE GLUCANPHOSPHORYLASE ACTION AT CULTIVATED AND SPONTANEOUS GRAMINACEAE
CIORNEA ELENA 1, DUMITRU GABRIELA 2 1. "Alexandru Ioan Cuza" University of Iasi, Carol I Bvd., 20 A, Faculty of Biology, Laboratory of Biochemistry and Molecular Biology, Romania e-mail: ciornea@uaic.ro 2. "Alexandru Ioan Cuza" University of Iasi, Carol I Bvd., 20 A, Faculty of Biology, Laboratory of Biochemistry and Molecular Biology, Romania e-mail: gabriela.dumitru@uaic.ro
The biological material was represented by germinated caryopses of graminaceae derived from the cultivated and spontaneous flora, namely millet (Panicum miliaceum), sorghum (Sorghum vulgare), Sudan grass (Sorghum sudanense), hair grass (Festuca pratensis), bristle grass (Setaria pumila) and brome grass (Bromus sterilis), determining the starch mobilization rate, as the main reservesubstance, under the phosphorolytical action of αglucanphosphorylase, in parallel with the concentration of the total soluble proteins. The germination of seeds was achieved in laboratory conditions along ten days of germination, the sampling being performed every 24 hours. The analysis of the obtained results for all analyzed species underlined the existence of a strong correlation between the mobilization degree of the reserve substances and the enzymatic activity, noticing in this sense an intensification of the enzymatic activity at the decrease of starch concentration.
