In order to determine the methanol
concentration, the biological samples (blood,
tissues) were analyzed by an Agilent
Technologies 7890 gas chromatograph with an
Agilent Technologies 7683 Series automatic
injector equipped with a Zebron – Phenomenex,
ZB-WAX plus column (60 m x 0.25 mm, 0.25
µm), and Agilent Technologies 5975C inert MSD
detector; data acquisition was made by SIM
method. Data interpretation: Agilent
Technologies ChemStation software. The
temperature program begins from 50°C (constant
for 16 minutes), after that. the temperature
increase with 25°C/min up to 250°C, the mobile
phase (He) with a debit of 1 ml/min. The source
temperature is 230°C and the quadruple’s is 150
°C. In these conditions. the method is linear in the
5 – 20000 μg/mL range. the detection limit is
417.8 ÎĽg/mL, the quantification limits is 1266.1
μg/mL. There were determined: the system’s
precision (RSD = 0.8748%), method precision
(RSD = 4.6811%), intermediate precision (RSD =
4.1078 %); the accuracy was 98.0% in 93.2 –
102.8% range. Under these conditions, the
methanol concentration was determined from
biological samples, that is, blood and tissues. | 
