IN VITRO CULTURE OF UNFERTILIZED OVULES
IN BRASSICA OLERACEA L.
PRISECARU MARIA 1, CRISTEA TINA OANA 2, MARIA CÄLIN 3 1. Faculty of Sciences, Biology Deprtament, University âVasile Alecsandriâ , Marasesti Street no. 157, Bacau, Romania, e-mail: prisecaru_maria@yahoo.com
2,3 Vegetable Research and Development Station Bacau, calea Barladului no. 220, Romania, e-mail: tinaoana@yahoo.com; e-mail: sclbac@legumebac.ro
Using the unfertilised ovule culture of 4 genotypes of Brassica oleracea var. capitata (three consanguine lines and one hybrid) haploid plants diploid and mixoploid plants with gynogenetic origin were successfully obtained. A larger number of embryos/callus/ovules and normal plants were obtained. The gynogenesis in vitro through unfertilised ovule culture can be an effective method of production of haploids and dihaploids at white cabbage.
