In order to investigate whether the type of carbohydrate used as a carbon source might affect the efficiency of regeneration, a complete screening of the main types of carbohydrates in different concentrations were tested. The study was conducted at Vegetable Research and Development Station Bacau, in The Laboratory of Tissue Culture. The explants were collected from valuable mother plants maintained at Vegetable Research Station Bacau in controlled conditions. The results obtained demonstrate without doubt that among the different carbohydrates used, maltose is the most efficient in inducing multiple shoot number, followed by sucrose, glucose and fructose. The maximum mean shoot number (22.0 ± 0.10) was recorded at 3% maltose, with maximum frequency of shoot regeneration (78%). Sucrose also obtained very good results, as it promoted a shoot regeneration frequency of 70%, with a maximum nember of shoots per explant of 19.20 ± 0.16.
