THE STUDY OF COMPOSITION IN SOLUBLE SUGARS AT HAPLOID GAMETOCLONES WITH GYNOGENETIC ORIGIN
MARIA PRISECARU 1, IONUŢ STOICA 1, GABRIEL ALIN IOSOB 2, MARIA CĂLIN 3 1. „Vasile Alecsandri” University of Bacau, Faculty of Science, Department of Biology, Ecology and Environment Protection, Marasesti Street, no. 157, Romania, e-mail: prisecaru_maria@yahoo.com;ionut_stoica23@yahoo.com
2. Doctoral School - „Vasile Alecsandri” University of Bacau, Marasesti Street, No 157, Bacau, Romania, e-mail: iosob.gabriel@gmail.com;
3. Vegetable Research and Development Station Bacau, Calea Barladului, No. 220, Bacau, code: 600388
The soluble sugars that are present in the haploid plants of 10-12 cm length obtained by gynogenesis „in vitro” via the culture of nonfertilized ovaries and ovules have been identified and quantitatively measured in wheat (Triticum aestivum L.), sugarbeet (Beta vulgaris L.), sunflower (Helianthus annuus L.) and potato (Solanum tuberosum L.) by using the chromatographic method upon thin larger and UV-VIS spectrophotometry. Diploid plants of the same length where used for control. They were obtained by germination of the donor plants seeds from where ovaries and ovules for cultivation in vitro were sampled. Thus, it is found that in the haploids obtained in vitro, for all studied species, the values of sugars are in general clearly low, as compared to the control diploid plants. The haploid plants chromatograms show also the lack of some sugars identified in the control diploid plants. It is interesting to note that in some haploids new sugars appear, but they are not identified in the 2n plants, or in almost equal quantitative values, or higher that the 2n donor plant value (case of sugar beet).
